If the bacteria was extremely sensitive to low oxygen, then it might not grow in pour plates because of the slightly reduced levels as you go deeper in the agar.
Could bacteria grow on the streak plate and not be seen using the pour plate technique?
I eat steak wunst in a great while. And I always eat it off a clean plate. I pour BarbieQ sauce on it. The plate gets sauce stains on it, of course.
Reply:Obligate anarobes do not grow well in pour plate techniques. There is one reason.